Tuberculosis Antibody ELISA Test Kit for Bovine Sheep 96Wells/Kit
ISO9001
1. TB Ab ELISA Test Kit Introduction
The Mycobacterium tuberculosis(Mycobacterium Bovis) antibody ELISA
kit is used to test Mycobacterium tuberculosis antibody in bovine
serum. It is used to evaluate the serologic assistant diagnosis of
tuberculosis caused by Mycobacterium bovis.
This kit use indirect ELISA method, Mycobacterium tuberculosis
antigen is pre-coated on enzyme micro-well strips. When testing,
add diluted serum sample, after incubation, if there is
Mycobacterium tuberculosis specific antibody, it will combine with
the pre-coated antigen, discard the uncombined antibody and other
components with washing; then add enzyme labled anti-Mycobacterium
tuberculosis antibody, it will combine with the complex of antibody
and pre-coated antigen on plate; discard the uncombined enzyme
conjugate with washing; Add TMB substrate in micro-wells, blue
product formed enzymatically, use ELISA reader at 450nm wavelength
to measure the absorbance A value in reaction wells after adding
stop solution to stop the reaction.
2. Reagents and contents
| Code | Item | Spec. | Code | Item | Spec. |
| 1 | Tuberculosis-Ag Coated plates 96wells | 1 plate | 7 | Stop solution | 12 ml |
| 2 | Enzyme Conjugate | 22 ml | 8 | Negative control | 2 ml |
| 3 | 10X Concentrated washing buffer | 50 ml | 9 | Positive control | 1.6 ml |
| 4 | Substrate | 12 ml | 10 | Adhesive Foil | 2 pieces |
| 5 | Sample diluent | 50 ml | 11 | Instruction sheet | 1 piece |
| 6 | Serum dilution plate | 1 piece | | | |
3. Material required not provided
1) Micropipette: 0.5µl-10µl, 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells.
5) Distilled water or deionized water.
6) Microplate Washer
4. TB Ab ELISA Test Kit Sample preparation
Take animal whole blood, get serum by using regular method, the
serum should bright and no hemolysis
5. Washing buffer preparation
Return 10X Concentrated washing buffer into room temperature before
use, if there is salt crystals, shake to make it dissolved, then
dilute it at 10 times with distilled water or deionized water. The
diluted washing buffer can store at 4℃ for about 1 week.
6. Sample dilution
Dilute the serum sample with Sample diluent at 1:99(for example:
495ul sample diluent + 5ul serum sample)
Notice: Negative control and Positive control do not need dilute.
Exchange tip after taking sample every time, record the position of
the sample on plate accurately. Shake the sample evenly before
adding it.
7. Notes
1) Return all reagents into room temperature before use, shake it
evenly before use, and store back to 2-8℃ after usage.
2) Do not mix use reagents from different kits and different lot
no., prevent the reagents been polluted when using.
3) Substrate and stop solution may have irritation to skin and
eyes, be careful to use.
4) Do not expose Substrate to strong light and avoid contact with
the oxidant.
5) Tuberculosis-Ag coated plates should be sealed and
moisture-proof. Put back unused MicroWell plate into dry foil bag
and sealed at 2~8 ℃.
6) All wastes should be treated well to avoid pollution before
discarding.
7) Strict compliance with the operating instructions can get the
best results. Pipetting operation, timing, and washing of the whole
process must be precise.
8) Serum dilution plate is disposable, do not repeat use. The
maximum capacity of the serum dilution plate was 300ul/well.
8. Test procedure
1) Take Tuberculosis-Ag Coated plates (open and take the quantity
required for use according to sample quantity), add diluted serum
into test wells, 100ul/well; meanwhile set 2 wells for positive
control and 1 well for negative control, add negative control and
positive control into its wells accordingly, 100ul/well(do not
spill);
2) Cover it with Adhesive Foil, incubate at 37 ℃ for 30 minutes;
3) Open the adhesive foil, discard the liquid of the well, add
diluted washing buffer to each well, 250ul/well, discard the
liquid, repeat the above step for 4-6 times, at last flap to dry
with the absorbent paper;
4) Adding Enzyme Conjugate,100ul/well, shake gentle to mix it
evenly, cover it with Adhesive Foil, incubate at 37 ℃ for 30
minutes;
5) Open the adhesive foil, discard the liquid of the well, add
diluted washing buffer to each well, 250ul/well, discard the
liquid, repeat the above step for 4-6 times, at last flap to dry
with the absorbent paper;
6) Add substrate, 100ul/well, mix it evenly then cover it with
Adhesive Foil, incubate at 37 ℃ in dark for 10 minutes;
7) Add stop solution, 50ul/well to stop the reaction, measure the
result in 10 minutes.
9. Results judgement
Read the OD value at 450nm (630nm as reference).
For the assay to be valid:
OD value of negative control(N) < 0.2, meanwhile OD value of
positive control (P) > 0.4
Calculate method:
S/P value= Sample OD value/ Positive control OD average value
Results interpretation
S/P value < 0.3: Negative
S/P value)≥ 0.3: Positive
10. Storage and expire date
Store at 2~8℃ in dark, no frozen, expiry date: 12 months.
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific
needs and specific quantities.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so
far all the production process, we have standard rules, we comply
with the relevant behavior and laws of the government.
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service.
If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
Q5: What is your minimum order quantity?
A5: 1Kit.
Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by
air and ground.Please confirm with us before placing an order.
