Classical Swine Fever Virus Antibody Diagnostic ELISA Kit for Pig
192 Wells/Kit
1. Classical Swine Fever Virus Antibody Diagnostic ELISA Kit Principle
GreenSpring® Classical Swine Fever Virus (CSFV) Antibody ELISA
Detection Kit is made from antigen-coated microtiter plates (CSFV
antigen-coated) and other reagents. It uses the principle of
solid-phase ELISA to detect CSFV-Ab in serum, and then adds an
enzyme conjugate to specifically bind to the complex of coated
antigen + CSFV-Ab + enzyme-labeled anti-pig IgG antibody on the
microplate. With TMB substrates, a certain amount of color is
produced. The depth of color is related to the content of CSFV-Ab.
When the color value is greater than the critical value, the pig is
well inoculated or naturally infected.
2. Classical Swine Fever Virus Antibody Diagnostic ELISA Kit
Components
| 1 | CSFV antigen coated microplate | 96T X 2 |
| 2 | Enzyme conjugate | 22ml | yellow lid |
| 3 | Sample diluent solution | 50ml | transparent lid |
| 4 | CSFV-IgG Negative control serum | 1.5ml | green lid |
| 5 | CSFV-IgG Positive control serum | 1.5ml | red lid |
| 6 | Substrate | 12ml X2 | orange lid |
| 7 | Stop solution | 12ml | blue lid |
| 8 | 20×concentrated washing buffer | 50ml | white lid |
| 9 | Adhesive Foil | 2 pieces | |
| 10 | Instruction | 1 piece | |
3. Results
For the assay to be valid, the mean OD value of the positive
control wells must be greater than or equal to 1.0, and the mean OD
value of the negative control wells must be less than 0.1.
Otherwise, the test is invalid and needs to be re-tested.
The result is judged by the S/P value,
S/P=(sample OD450/630- NCx(—))/( PCx(—)- NCx(—)), NCx(—) represents
the average OD450/630 value of the negative control (less than
0.05, calculated as 0.05), PCx(— ) represents the mean OD450/630
value of the positive control
S/P≥0.25 is positive; less than 0.25 is negative.
4. User Notices and Warnings
1. This kit is for research use only.
2. Do not use expired reagents, and do not mix reagents from
different batches.
3. Please read the manual carefully before use.
4. The experimental garbage should be sterilized by high pressure
steam at 121℃ for 30 minutes, or treated with 5.0g/L sodium
hypochlorite disinfectant for 30 minutes and then discarded.
5. Microplates removed from refrigeration should be equilibrated
and allowed to dry at room temperature before opening. Return
unused MicroWell plates to dry foil bags and seal at 4 °C. Unused
liquid reagents should be covered and stored together with other
components at 2-8°C away from light.
6. Micropipettes should be used to add samples and reagents and
should be frequently demonstrated for accuracy.
7. When adding washing buffer, it should be topped up but not
overflowed to avoid free enzyme or cross-contamination between
wells.
8. The stop solution is corrosive, immediately rinse with plenty of
water when it comes into contact with skin or clothes.
Specifications: 96 holes × 2.
Validity: 12 months.
Storage: 2~8℃, keep away from light.
Production date: on the outer packaging of the kit.
Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7
working days after receiving the payment. (In case of external
factors such as the epidemic, the delivery may be delayed)
Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more
than 100,000 pieces, which is convenient for customized products.
Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our
production process conforms to standard procedures to ensure
optimum product quality.
Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service.
We can provide you with one-on-one guidance via video, telephone,
etc.
Q5: What is the payment method?
A5: We receive payment by T/T.
Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from
our many cooperative carriers, and also ship according to your
requirements.
