Swine Transmissible Gastroenteritis (TGE) Antibody ELISA Kit 192
Wells/Kit
1. Usage
This kit is used to detect swine Transmissible Gastroenteritis
(TGE) antibody in porcine serum.
2. Notes
1) All reagents should return to the room temperature(18~26℃)
before using, and store back at 2-8℃after using
2) Do not use kit out of expiry date. Do not mix use reagents from
kits of different lot numbers.
3) The unused micro-wells should seal back to bag and store back at
2-8℃.
4) Used materials should be treated innocuously, and be handled in
accordance with local, regional and national regulations.
5) Avoid Substrate exposure to bright light, avoid contact with
oxidants.
6) When the quantity of serum sample is big, dilute all serum
sample on serum dilute plate firstly, then transfer all diluted
serum sample on reaction wells, make the reaction time same.
7) Stop solution is corrosive, if splashed on the skin or clothing
should immediately rinse with plenty of water.
8) Strictly adhere to instruction to get best result. All procedure
including pipetting, timing and washing etc. must be accurate to
get accurate result.
3. The kit components
| 1 | TGE antigen coated micro-plate | 96T X 2 |
| 2 | Enzyme conjugate | 22ml | yellow lid |
| 3 | Sample diluent solution | 50ml | transparent lid |
| 4 | TGENegativecontrol serum | 1.5ml | green lid |
| 5 | TGEPositivecontrol serum | 1.5ml | red lid |
| 6 | Substrate | 12ml X2 | orange lid |
| 7 | Stop solution | 12ml | blue lid |
| 8 | 20×concentrated washing buffer | 50ml | white lid |
| 9 | Adhesive Foil | 2 pieces | |
| 10 | Instruction | 1 piece | |
4. Preparation
1) Sample dilute: Dilute sample with the sample diluent at 40
times.(5ul serum + 195ul sample diluent), the diluted sample need
to mix evenly to get better results. Negative control and positive
control do not need dilute.
2) Washing solution preparation: Dilute the 20×concentrated washing
buffer with deionized water or distilled water at 20 times. (10ml
20×concentrated washing buffer + 190ml deionized water ) It is
normal if there is crystallization in the 20×concentrated washing
buffer, put at 37℃until completely dissolved.
5. ELISA procedure
1) Take pre-coated microtiter strips (Can unseal for several time
use as per sample quantity), add 100μL diluted serum to test well,
meanwhile set 2 wells for Negative control, 2 wells for Positive
control separately. Add 100 μL Negative/Positive control to its
well. Shake softly, cover andincubate at 37℃ for 30 min.
2) Pour the liquid out of the wells, add diluted washing solution
to each well fully, be static for 10s, pour out. Wash3 times, at
last pat to dry on absorbent paper.
3) Add 100 μL Enzyme Conjugate to each well, andincubate at 37℃ for
30 min.
4) Repeat the step 2(washing). Remember pat to dry on absorbent
paper at last.
5) Add 100 μL substrate to each well, mix properly,react for 10 min
at 37℃ at dark.
6) Add 50 μL stop solution in each well, and measure the result
within 10 min(shake evenly before read result, no spill).
7) Read OD value with ELISA reader at 450nm/630nm.
6. Results
For the assay to be valid, the positive control wells’ average OD
value must be greater than or equal to 0.6, and the negative
control wells’ average OD value is less than 0.1. Otherwise the
test is invalid, need test again.
The result is judged by S/P value,
S/P=(Sample OD450/630- NCx(—))/( PCx(—)- NCx(—)), NCx(—) means
Negative control’s average OD450/630 value(calculate as 0.05 when
the value is less than 0.05), PCx(—) means Positive control’s
average OD450/630 value
If S/P≥0.2, it is positive; less than 0.2, it is negative.
Specifications: 96*2 wells/kit.
Expiry date:12 months.
Storage: Storing at 2-8℃, in the dark.
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific
needs and specific quantities.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so
far all the production process, we have standard rules, we comply
with the relevant behavior and laws of the government.
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service.
If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
Q5: What is your minimum order quantity?
A5: 1Kit.
Q6: What is the shipping method?
A6: Usually by air, we will choose the best shipping method for
you, and we can also ship according to the shipping method you
require.
