Porcine Epidemic Diarrhea (PED) Antibody ELISA Kit 192 Wells/Kit
ISO9001
1. Usage
This kit is used to detect Porcine Epidemic Diarrhea (PED) antibody
in porcine serum.
2. Notes
1) All reagents should be returned to room temperature (18~26℃)
before use, and stored at 2-8℃ after use
2) Do not use expired kits. Do not mix reagents from different
batches of kits.
3) Unused micropores should be sealed back in the bag and stored at
2-8°C.
4) Used materials should be disposed of harmlessly and in
accordance with local, regional and national regulations.
5) Avoid exposing the substrate to strong light and avoid contact
with oxidizing agents.
6) When the amount of serum samples is large, first dilute all
serum samples on the serum dilution plate, and then transfer all
the diluted serum samples to the reaction wells, so that the
reaction time is the same.
7) The termination liquid is corrosive, if it splashes on the skin
or clothes, it should be washed with plenty of water immediately.
8) Strictly follow the instructions for best results. All
procedures including pipetting, timing, and washing must be
accurate for accurate results.
3. The kit components
| 1 | PED antigen coated micro-plate | 96T X 2 |
| 2 | Enzyme conjugate | 22ml | yellow lid |
| 3 | Sample diluent solution | 50ml | transparent lid |
| 4 | PED Negative control serum | 1.5ml | green lid |
| 5 | PED Positive control serum | 1.5ml | red lid |
| 6 | Substrate | 12ml *2 | orange lid |
| 7 | Stop solution | 12ml | blue lid |
| 8 | 20×concentrated washing buffer | 50ml | white lid |
| 9 | Adhesive Foil | 2 pieces | |
| 10 | Instruction | 1 piece | |
4. Preparation
1) Sample dilute: Dilute sample with the sample diluent at 40
times.(5ul serum + 195ul sample diluent), the diluted sample need
to mix evenly to get better results. Negative control and positive
control do not need dilute.
2) Washing solution preparation: Dilute the 20×concentrated washing
buffer with deionized water or distilled water at 20 times. (10ml
20×concentrated washing buffer + 190ml deionized water ) It is
normal if there is crystallization in the 20×concentrated washing
buffer, put at 37℃until completely dissolved.
5. ELISA procedure
1) Take pre-coated microtiter strips (Can unseal for several time
use as per sample quantity), add 100μL diluted serum to test well,
meanwhile set 2 wells for Negative control, 2 wells for Positive
control separately. Add 100 μL Negative/Positive control to its
well. Shake softly, cover and incubate at 37℃ for 30 min.
2) Pour the liquid out of the wells, add diluted washing solution
to each well fully, be static for 10s, pour out. Wash3 times, at
last pat to dry on absorbent paper.
3) Add 100 μL Enzyme Conjugate to each well, and incubate at 37℃
for 30 min.
4) Repeat the step 2(washing). Remember pat to dry on absorbent
paper at last.
5) Add 100 μL substrate to each well, mix properly,react for 10 min
at 37℃ at dark.
6) Add 50 μL stop solution in each well, and measure the result
within 10 min(shake evenly before read result, no spill).
7) Read OD value with ELISA reader at 450nm/630nm.
6. Results
For the assay to be valid, the positive control wells’ average OD
value must be greater than or equal to 0.6, and the negative
control wells’ average OD value is less than 0.1. Otherwise the
test is invalid, need test again.
The result is judged by S/P value,
S/P=(Sample OD450/630- NCx(—))/( PCx(—)- NCx(—)), NCx(—) means
Negative control’s average OD450/630 value(calculate as 0.05 when
the value is less than 0.05), PCx(—) means Positive control’s
average OD450/630 value
If S/P≥0.2, it is positive; less than 0.2, it is negative.
Specifications: 96*2 wells/kit.
Expiry date:12 months.
Storage: Storing at 2-8℃, in the dark.
Pathogenesis of porcine epidemic diarrhea virus
The virus enters the small intestine directly after oral and nasal
infection. By immunofluorescence and electron microscopy,
replication of the virus was carried out in the cytoplasm of the
villous epithelium of the small intestine and colon. No virus
proliferation was found in other organs. The proliferation of virus
first causes damage to organelles, and then causes dysfunction of
fine cells. The atrophy of intestinal villi leads to the reduction
of absorption surface area, and the significant reduction of
alkaline phosphatase content in small intestinal mucosa leads to
nutrient absorption disorder, which is the main cause of diarrhea
and belongs to osmotic diarrhea. Dehydration caused by severe
diarrhea is the main cause of death of sick pigs.
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific
needs and specific quantities.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so
far all the production process, we have standard rules, we comply
with the relevant behavior and laws of the government.
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service.
If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
Q5: What is your minimum order quantity?
A5: 1Kit.
Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by
air and ground.Please confirm with us before placing an order.
